The Journal of International
Advanced Otology
Original Article

Angiotensin-Converting Enzyme (ACE) I/D and Alpha-Adducin (ADD1) G460W Gene Polymorphisms in Turkish Patients with Severe Chronic Tinnitus


Department of Otorhinolaryngology, Cumhuriyet University School of Medicine, Sivas, Turkey.


Department of Biochemistry, Cumhuriyet University School of Medicine, Sivas, Turkey


Department of Medical Genetics, Cumhuriyet University School of Medicine, Sivas, Turkey


Department of Ear Nose Throat Head and Neck Surgery, Antalya Training and Research Hospital, Antalya, Turkey.


Department of Nutrition and Dietetics, Cumhuriyet University School of Health Sciences, Sivas, Turkey

J Int Adv Otol 2016; 12: 77-81
DOI: 10.5152/iao.2016.1732
Read: 638 Downloads: 217 Published: 03 September 2019


OBJECTIVE: Tinnitus is described as a disturbing sound sensation in the absence of external stimulation. We aimed to investigate whether there is any relationship between severe chronic tinnitus and angiotensin-converting enzyme (ACE) I/D and α-adducin (ADD1) G460W gene polymorphisms.

: The patient group and control group consisted of 89 and 104 individuals, respectively. The evaluation of tinnitus was performed using the Strukturiertes Tinnitus-Interview (STI). The Tinnitus Handicap Inventory (THI) was used to evaluate the tinnitus severity. Polymerase chain reaction (PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques were used for genotyping.

: With regard to the ACE I/D polymorphism, there was no significant difference in genotype and allele frequencies between the patient group and control group. However, a statistically significant difference was found in genotype (p<0.01) and allele frequencies (p=0.021) of the ADD1 G460W gene polymorphism. Combined genotype analysis showed that the ACE II /ADD1 GW genotype was statistically significantly higher in the patient group than in the control group (X2: 7.15, p=0.007). The odds ratio value of the GW genotype was 2.5 (95% CI=1.4–4.7) (p<0.01).


CONCLUSION: Our results demonstrate an association between ADD1 G460W gene polymorphism and susceptibility to severe chronic tinnitus. It was found that the GW genotype increased the disease risk by 2.5-fold compared with other genotypes. This indicates that ADD1 G460W polymorphism could be an important factor in the pathophysiology of tinnitus.

EISSN 2148-3817