The Journal of International
Advanced Otology
Original Article

Mammalian Cochlear Hair Cell Imaging Using Optical Coherence Tomography (OCT): A Preliminary Study

1.

Department of Otorhinolaryngology and Pusan National University School of Medicine, Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea

2.

Koh Young Technology Inc., Geumcheon-gu, Seoul, Republic of Korea

J Int Adv Otol 2021; 17: 46-51
DOI: 10.5152/iao.2020.8377
Read: 178 Downloads: 62 Published: 29 January 2021

OBJECTIVES: This study aimed to investigate the feasibility of using optical coherence tomography (OCT) to provide information about cochlear microanatomy at a cellular level, specifically of cochlear hair cells in mammals.

MATERIALS AND METHODS: A total of 10 Sprague-Dawley rats were divided into 2 experimental groups for comparing the arrangement of normal and damaged hair cells. Postnatal day 3 Sprague-Dawley rats were used to test the swept-source OCT system, and the images recorded were compared with fluorescence microscope images.

RESULTS: Intracochlear structures (the inner hair cells, outer hair cells, and auditory nerve fibers) were clearly visualized at the individual cellular level.

CONCLUSION: These images reflect the ability of OCT to provide images of the inner hair cells, outer hair cells, and auditory nerve fibers (ex vivo). OCT is a promising technology, and these findings could be used to encourage research in the area of cochlear microstructure imaging in the future.

Cite this article as: Choi SW, Kang J, Lee S, Oh SJ, Kim H, Kong SK. Mammalian Cochlear Hair Cell Imaging Using Optical Coherence Tomography (OCT): A Preliminary Study. J Int Adv Otol 2021; 17(1): 46-51.

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