The Journal of International Advanced Otology
Original Article

The Effect of Nystatin Solution on Otoacoustic Emissions in Rats


Department of Otorhinolaryngology, Erciyes University School of Medicine, Kayseri, Turkey


Department of Otorhinolaryngology, Kayseri Training and Research Hospital, Kayseri, Turkey


Department of Otorhinolaryngology, Private Maya Hospital, Kayseri, Turkey


Department Of Otorhinolaryngology, Kemerburgaz University School of Medicine, İstanbul, Turkey

J Int Adv Otol 2017; 13: 105-109
DOI: 10.5152/iao.2016.1667
Read: 229 Downloads: 49 Published: 03 September 2019


OBJECTIVE: In patients with a perforated tympanic membrane, topically administered medication reaches the middle ear and thus creates a risk of ototoxicity. The aim of the present study was to evaluate the possible ototoxic effect of the antifungal medication nystatin when administered to the rat middle ear cavity.


MATERIALS and METHODS: Three groups (negative control, positive control, and study groups), each containing eight rats, were formed. Before the drug administration, distortion product otoacoustic emissions were recorded in both ears of each rat. Saline (0.09% NaCl), gentamycin, and nystatin solutions were transtympanically injected into the middle ear cavities of the negative control, positive control, and study groups, respectively, for five consecutive days. Seven days after the last infiltration, the control otoacoustic emission was measured, and the data of the 2, 3, 4, 6, 8 kHz frequencies were statistically analyzed.


RESULTS: There were no significant changes between the 1st and 2nd measures in the negative control group (0.09% NaCl) (p>0.05), whereas there were significant changes between the 1st and 2nd measures in the positive control group (gentamycin) and study group (nystatin) (p<0.05).


CONCLUSION: Ototopical medications carry a risk of ototoxicity in patients with perforated ear drums. In the present study, it was shown that nystatin, an antifungal that can be ototopically used in the treatment of otomycosis, may cause a decrease in otoacoustic emissions in rats when administered into the middle ear cavities. 

ISSN1308-7649 EISSN 2148-3817