The Journal of International
Advanced Otology
Original Article

RhoA, ROCK-1, and ROCK-2 Gene Expression and Polymorphisms in Cholesteatoma Patients

1.

Department of Otorhinolaryngology Faculty of Medicine, University of Mersin, Mersin, Turkey

2.

Department of Pharmacology, Faculty of Medicine, University of Mersin, Mersin, Turkey

3.

Department of Medical Biology and Genetics Faculty of Medicine, University of Mersin, Mersin, Turkey

J Int Adv Otol 2021; 17: 530-535
DOI: 10.5152/iao.2021.21132
Read: 213 Downloads: 66 Published: 16 December 2021

BACKGROUND: The aim of this study was to evaluate the gene polymorphism and expressions of Rho-A, ROCK-1, and ROCK-2 in cholesteatoma.
METHODS: In this study, 120 healthy control group patients and 120 cholesteatoma patients were enrolled. Venous blood was taken from all of the cholesteatoma and control group patients. The genotyping of ROCK-1(G/T)rs35996865, ROCK-2(A/C)rs10178332, and Rho-A(A/T)rs2177268 polymorphisms was performed using predesigned TaqMan SNP Genotyping Assays. Assays-on-Demand SNP genotyping kit was used for the realtime polymerase chain reaction. The expression levels of Rho-A(Hs00357608_m1), ROCK-1(Hs01127699_m1), and ROCK-2(Hs00178154_m1) genes were determined.
RESULTS: The expression of Rho-A, ROCK-1, and ROCK-2 was lower in cholesteatoma patients than in the control group. There was no difference in Rho-AAT/TT and ROCK-1GT/TT variation in cholesteatoma patients compared to the control. However, ROCK-2 AC/CC variance was lower in cholesteatoma patients.
CONCLUSION: The expression of Rho-A, ROCK-1, and ROCK-2 genes may be decreased in cholesteatoma. Furthermore, since ROCK-2 AC/CC genotype is also lower in cholesteatoma, having C allele seems to decrease the risk of developing this disease.
Cite this article as: Görür K, Büyükafşar K, Akbaş E, et  al. RhoA, ROCK-1 and ROCK-2 gene expression and polymorphisms in cholesteatoma patients. J Int Adv Otol. 2021;17(6):530-535.

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